Journal: eNeuro
Article Title: The Auditory Brainstem Response Diagnoses Alzheimer-Like Disease in the 5xFAD Mouse Model
doi: 10.1523/ENEURO.0049-25.2025
Figure Lengend Snippet: No differences in peripheral neuronal structures were observed between genotypes. A , Representative view of SGN labeling in 5xFAD and WT mice at 12M. Images show the 16 kHz regions, with labels for DAPI (blue), Calb2 (green), and TUJ1 (red). B , Quantification of Type 1 SGN density (left) and the proportion of Type 1c SGNs (right). C , Representative view of afferent and efferent terminal labeling in cochlear whole mounts from 5xFAD and WT mice at 12M, with labels for CtBP2 (green) and VAT (magenta). Tonotopic cochlear regions for 8 kHz (left column) and 16 kHz (right column) are shown. D , Quantification of CtBP2 puncta number per IHC (left) and mean VAT terminal volume per IHC (right). E , Representative view of efferent terminal labeling (VAT in white color) in cochlear whole mounts from 5xFAD and WT mice at 12M. The 8 (top row), 16 (middle row), and 32 (bottom row) kHz regions are shown. F , Quantification of VAT terminal volume (µm 3 ) per OHC. Asterisks denote the significance of differences between WT and 5xFAD mice: no significance (ns), p ≥ 0.05; * p < 0.05; ** p < 0.01; and *** p < 0.001, Student's t test or Wilcoxon test. Scale bar, 10 μm for A , C , and E .
Article Snippet: The following primary antibodies were used in this analysis: mouse TUJ1 (anti-beta Tubulin 3, 1:100, R&D Systems) and mouse anti- calbindin 2 (Calb2, 1:100, Millipore Sigma).
Techniques: Labeling